Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice

The high cost of chemical pesticides and their negative impact on the environment prompted search for natural from plants. objective our study was to control rice seed pathogenic fungi bacteria using aqueous extract essential oil Tithonia diversifolia leaves. We obtained oil, respectively, by maceration hydrodistillation; antimicrobial activities were determined in vitro a solid medium food poisoning method. secondary metabolites qualitative quantitative assays; composition Titonia studied gas-chromatography coupled with mass spectrometry. results showed that phenols, tannins, flavonoids, alkaloids, terpenoids, sugars saponins present extract. contained mainly hydrocarbonated, oxygenated monoterpenes, terpenoids sesquiterpenes. ?-terpineol (20.3%), eucalyptol (14.6%), camphor (14.3%) ?-pinene (13.5%) as main compounds. Regarding activity, all tested sensitive oil. activity an inhibitory concentration 50 (IC50) mg/mL against Bipolaris oryzae Fusarium moniliforme. MIC 125 ?g/mL (Xanthomonas pv. Pseudomonas fuscovaginae) MFC 5000 (Bipolaris moniliforme). These allow us consider potential source biopesticides seed-borne pathogens.